Investigation of ovarian cancer associated sialylation changes in N-linked glycopeptides by quantitative proteomics

Shetty, Vivekananda; Hafner, Julie; Shah, Punit; Nickens, Zacharie; Philip, Ramila

doi:10.1186/1559-0275-9-10

Clinical Proteomics

Table 1 List of identified glycosylation sites and the results of quantitation of N-deglycosylated peptides

From: Investigation of ovarian cancer associated sialylation changes in N-linked glycopeptides by quantitative proteomics

Swissprot ID	Protein	Glycopeptide sequence	Glycosylation site	Charge	Observed mass (Da)	Molecular weight (Da)		Δ mass (Da)	Mascot score	Heavy/Light Ratio (Peptide)a	Mean ratio (Protein)
						Mr(expt)	Mr(calc)
*Proteins with increase in sialylation*
P01009	Alpha-1-antitrypsin ^†										2.1
		YLGNAT AIFFLPDEGK^*	271	2	902.29	1802.57	1802.90	-0.33	88	2.1
O75882	Attractin^†										2.2
		NHS CSEGQISIFR^*	731	2	791.71	1581.41	1581.71	-0.31	54	2.2
P00738	Haptoglobin^†										2.4
		VVLHPNYS QVDIGLIK^*	241	2	941.60	1881.18	1881.01	0.17	51	2.7
		NLFLNHS ENAT AK	207,211	2	775.05	1548.08	1547.72	0.36	68	2.0
P02787	Serotransferrin^†										5.4
		CGLVPVLAENYNK^*	432	2	763.12	1524.23	1523.76	0.47	88	7.6
		QQQHLFGSNVT DCSGNFCLFR^*	630	2	1281.94	2561.86	2562.13	-0.27	44	3.1
P27169	Serum paraoxonase/arylesterase 1 (PON1)^†										4.0
		HANWT LTPLK^*	253	2	614.82	1227.63	1227.65	-0.03	58	4.0
P04114	Apolipoprotein B-100^†										1.5
		FVEGSHNST VSLTTK^*	3411	2	827.75	1653.48	1653.81	-0.33	91	2.0
		YDFNSS MLYSTAK	3465	2	795.71	1589.41	1589.68	-0.27	68	1.0
P02790	Hemopexin^†										1.9
		ALPQPQNVTSLLGCTH ^*	453	2	897.77	1793.52	1793.88	-0.36	83	2.1
		SWPAVGNCS SALR	187	2	725.38	1448.75	1448.66	0.09	83	1.6
P25311	Zinc-alpha-2-glycoprotein^†										1.5
		FGCEIENNR	128	2	592.57	1183.14	1182.48	0.65	71	0.9
		DIVEYYNDS NGSHVLQGR^*	109	2	1057.82	2113.63	2112.96	0.67	54	2.0
*Proteins with increase and decrease in sialylation*
P00450	Ceruloplasmin^†										1.8
		EHEGAIYPDNTT DFQR^*	138	2	971.00	1939.99	1939.85	0.14	91	3.1
		AGLQAFFQVQECNK	358	2	864.07	1726.13	1725.79	0.35	99	0.4
		ENLT APGSDSAVFFEQGTTR	397	2	1088.42	2174.84	2174.01	0.83	74	1.9
*Proteins with no change in sialylation*
P02763	Alpha-1-acid glycoprotein 1										1.8
		QDQCIYNTT YLNVQR	93	2	982.56	1963.11	1962.90	0.20	78	1.8
P19652	Alpha-1-acid glycoprotein 2										1.0
		QNQCFYNSS YLNVQR	93	2	985.07	1968.12	1967.87	0.25	79	1.0
P01011	Alpha-1-antichymotrypsin										1.3
		YTGNAS ALFILPDQDK	271	2	927.47	1852.93	1852.89	0.04	73	1.3
P02765	Alpha-2-HS-glycoprotein										1.4
		VCQDCPLLAPLNDT R	156	1	1816.60	1815.59	1815.83	-0.24	56	1.3
		KVCQDCPLLAPLNDT R	156	2	997.06	1992.10	1991.98	0.12	68	1.1
		AALAAFNAQNNGS NFQLEEISR	176	2	1207.45	2412.88	2412.16	0.72	107	1.6
P01008	Antithrombin-III										1.8
		SLTFNET YQDISELVYGAK	187	2	1136.45	2270.88	2270.10	0.78	53	1.8
P10909	Clusterin										1.1
		LANLTQGEDQYYLR	374	2	864.74	1727.47	1727.82	-0.35	80	1.1
P00748	Coagulation factor XII										1.4
		NHS CEPCQTLAVR	433	2	808.78	1615.55	1615.69	-0.14	69	1.4
P02749	Beta-2-glycoprotein 1										0.8
		VYKPSAGNNS LYR	162	2	778.62	1555.22	1554.75	0.47	45	1.1
		LGNWS AMPSCK	253	2	680.39	1358.77	1358.60	0.17	56	1.4
P08603	Complement factor H										1.4
		MDGASNVT CINSR	1029	2	745.00	1487.99	1487.63	0.36	49	1.0
		IPCSQPPQIEHGTINSS R	882	2	1033.76	2065.51	2064.97	0.54	90	1.9
P36980	Complement factor H-related protein 2										1.8
		LQNNENNIS CVER	126	2	819.61	1637.20	1636.74	0.46	58	1.8
P08185	Corticosteroid-binding globulin										1.6
		AQLLQGLGFNLT ER	96	2	804.87	1607.73	1606.86	0.87	74	1.6
O75636	Ficolin-3										1.1
		VELEDFNGNR	189	2	621.04	1240.06	1239.57	0.50	66	1.1
Q08380	Galectin-3-binding protein										1.5
		ALGFENAT QALGR	69	2	696.82	1391.63	1391.69	-0.06	66	1.5
P05546	Heparin cofactor 2										1.7
		NLS MPLLPADFHK	49	2	785.62	1569.22	1568.81	0.41	60	1.7
P19823	Inter-alpha-trypsin inhibitor heavy chain H2										1.0
		GAFISNFS MTVDGK	118	2	781.10	1560.18	1559.70	0.48	93	1.0
P03952	Kallikrein										1.2
		IYSGILNLS DITK	453	2	762.29	1522.57	1522.80	-0.23	90	1.2
P01042	Kininogen-1										1.2
		LNAENNAT FYFK	294	2	740.53	1479.05	1478.70	0.35	80	1.2
		ITYSIVQTNCS K	205	2	731.20	1460.38	1460.71	-0.33	64	1.6
		YNSQNQS NNQFVLYR	48	2	967.71	1933.40	1932.88	0.52	0.52	0.9
P05155	Plasma protease C1 inhibitor										1.3
		DTFVNAS R	238	2	479.10	956.18	956.45	-0.27	38	1.3
P00734	Prothrombin										1.8
		NFT ENDLLVR	416	2	634.62	1267.22	1267.63	-0.41	45	1.8
P04004	Vitronectin										1.2
		NGS LFAFR	169	2	485.67	969.32	969.48	-0.16	53	1.2
*Proteins with dicrease in sialylation*
P0C0L4	Complement C4-A										0.7
		GLNVT LSSTGR	1335	2	575.20	1148.39	1148.59	-0.20	72	1.2
		NTT CQDLQIEVTVK	1391	2	875.54	1749.07	1748.83	0.24	72	0.3

List of identified glycosylation sites and the results of quantitation of N-deglycosylated peptides obtained from normal and ovarian cancer sera by using LTL quantitative proteomics method.
^* The RSD of this method is 9% as determined by the reproducibility studies with fetuin [28].
^†* The ratio change in the glycopeptides of these proteins is due to increase in sialylation and not due to the increase in protein concentration.

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ISSN: 1559-0275

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