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Table 1 Systematic evaluation on performances of urine preparation methods

From: Syringe-push membrane absorption as a simple rapid method of urine preparation for clinical proteomics

  Nitrocellulose-SPMA Ultrafiltration Dialysis/lyophilization 75 % methanol precipitation
Spot number mean ± SD (inter-CV) 197 ± 15 (7.6 %) 196 ± 15 (7.6 %) 192 ± 17 (8.8 %) 199 ± 17 (8.4 %)
%Yield mean ± SD (inter-CV) 41.9 ± 2.6 (6.4 %) 42.9 ± 0.6 (1.5 %) 62.1 ± 8.4 (13.6 %) 55.6 ± 7.0 (12.6 %)
Need of specialized/high-cost instrument No No Yes (lyophilizer) Yes (superspeed centifuge)
Working time (min) 15 35 >1440 65
Step of procedure 3 3 3 5
Unit cost (US$/5-ml urine) 0.3 6.3 0.3 1.8
  1. Abbreviation: inter-CV inter-assay coefficient of variation