Fig. 4

Immunoblot analysis of the tissue-specific expression of the glial fibrillary acidic protein GFAP. Shown is a silver-stained gel (a) and corresponding immunoblots labelled with antibodies to the nuclear envelope protein lamin B1 (b), the cytosolic enzyme lactate dehydrogenase (c; LDH) and the glial fibrillary acidic protein (d; GFAP). Lanes 1–5 contain crude extracts from mouse liver, diaphragm, heart, leg muscle and brain, respectively. While lamin and lactate dehydrogenase were detected in all five tissues studied, GFAP could only be detected in brain samples, demonstrating the tissue-specific recognition of this protein by the antibody ab7260 to GFAP. Antibody labelling of increasing amounts of mouse brain protein per gel lane showed that a broader immuno-labelled GFAP banding pattern occurs at higher protein amounts (e). Lanes 6–13 contained 0, 2, 5, 10, 20, 40, 60 and 80 μg protein/lane, respectively