Fig. 5

Comparative immunoblot analysis of the glial fibrillary acidic protein GFAP in normal versus mdx-4cv brain. Shown are representative silver-stained gels (a, f, i) and immunoblots (b, c, g, h, j, k). Panels a–c contain 12-month old wild type (wt) and mdx-4cv brain specimens, and panels f–k show 2-, 8- and 12-month old samples. Blots were labelled with antibodies to lactate dehydrogenase (LDH as loading control) (b, g, j), and the glial fibrillary acidic protein (GFAP) (c, h, k). Arrowheads mark the main immuno-labelled protein bands in individual panels. Graphical representations of the immuno-decoration levels for LDH and GFAP in 12-month old wt versus mdx-4cv brain are shown in panels d and e: Student’s t test, unpaired; n = 4; *p < 0.05