Table 1 Operational characteristics of the Simple Plex device in comparison with peer immunoassay systems
From: A microfluidic multiplex proteomic immunoassay device for translational research
Method | ELISA | Bio Plex | Multi-array | Simple Plex |
|---|---|---|---|---|
Manufacturer | Bio-Techne | BioRad | Mesoscale discovery | ProteinSimple |
Number of analytes | Single analyte | Up to 100 anaytes | Up to 12 analytes | Up to 8 analytes |
Format | 96-well plate | 96-well plate | 96-well plate | Cartridge pre-filled with reagents |
Detection | Absorbance | Fluorescence | Electrochemiluminescence | Fluorescence |
Capacity (# samples/run) | 96 | 96 | 96 | 16 |
Assay time | 4–6 h/run | 4-6 h/run | 4-6 h/run | 1.5 h/run |
Level of automation (low, medium, high) | Low | Medium | Medium | High |
Volume needed | 600 µLa | 25 µL | 25 µL | 25 µL |