Skip to main content
Fig. 1 | Clinical Proteomics

Fig. 1

From: Targeting LAMP2 in human cerebrospinal fluid with a combination of immunopurification and high resolution parallel reaction monitoring mass spectrometry

Fig. 1

Validation of immunoprecipitation and identified LAMP2 peptides in CSF. a By employing Western blotting LAMP2 was detected when CSF had been immunoprecipitated with an anti-LAMP2 selective antibody, lane 1, and was found to migrate at approximately 80 kDa. LAMP2 was not precipitated using mouse serum IgG antibodies, lane 2. The detection was neither a result of unspecific binding of the secondary antibody as seen in the right panel. MW molecular weight ladder. b Shown is the sequence and theoretical structure of LAMP2A (UniProtKB:P13473) as indicated; signal peptide, aa 1–28; hinge region, aa 193–228; transmembrane region, aa 376–399; cytoplasmic tail, aa 400–410. Furthermore, the positions of the tryptic LAMP2 peptides identified by nano-LC–MS/MS are indicated

Back to article page

Clinical Proteomics

ISSN: 1559-0275