Table 1 Overview of proteomic studies of lung injury performed in the pediatric population over the last 10 years

References

Tsai et al. [24]

Neumann et al. [25]

Frischer et al. [27]

MacGregor et al. [29]

Sloane et al. [26]

Magagnotti et al. [15]

Participant age

1.9–4.8 years

2.1–15.3 years

9–28 years

5.5–7.1 years

9–13 years

25.3–31.1 days

Sample type (pretreatment)

Serum (no pretreatment)

BAL (protease inhibitor)

Bronchial tissue (extracted in sample buffer)

BAL (no pretreatment)

Sputum (DTT)

BAL (protease inhibitor)

Sample number

14 infected, 7 controls

10 infected, 7 controls

9 CF, 8 controls

39 CF, 38 controls

7 CF, 5 controls

9 BPD, 3 controls

Method employed

2-DE, MALDI-TOF

2-DE

MALDI-TOF, MALDI-TOF/TOF-MS

SELDI-TOF MS

MALDI-TOF

nLC-ESI-MS/MS

Further validation employed

Yes (ELISA, western blot)

No

No

Yes (Calgranulin A confirmed by western blot)

Yes (ELISA and western blot)

Yes (western blot)

Identified proteins

↑ haptoglobin,

↑ immunoglobulin kappa C,

↓ apolilipoprotein A-I,

↓ transthyretin

↑ α1-antitrypsin,

↓ transthyretin

↑ GRP75, mitochrondrial,

↑ Ubiquinol-cytochrome C reductase,

↑ Nidogen

↑ Calgranulin A,

↑ Calgranulin B,

↑ Calgranulin C

↑ α1-antitrypsin,

↑ myeoloperoxidase

↑ SFTP-A2,

↑ annexin-3,

↓ CLIC1,

↓ Calcyphosine