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Table 1 Overview of proteomic studies of lung injury performed in the pediatric population over the last 10 years

From: The proteomics of lung injury in childhood: challenges and opportunities

  Infection Cystic fibrosis BPD
References Tsai et al. [24] Neumann et al. [25] Frischer et al. [27] MacGregor et al. [29] Sloane et al. [26] Magagnotti et al. [15]
Participant age 1.9–4.8 years 2.1–15.3 years 9–28 years 5.5–7.1 years 9–13 years 25.3–31.1 days
Sample type (pretreatment) Serum (no pretreatment) BAL (protease inhibitor) Bronchial tissue (extracted in sample buffer) BAL (no pretreatment) Sputum (DTT) BAL (protease inhibitor)
Sample number 14 infected, 7 controls 10 infected, 7 controls 9 CF, 8 controls 39 CF, 38 controls 7 CF, 5 controls 9 BPD, 3 controls
Method employed 2-DE, MALDI-TOF 2-DE MALDI-TOF, MALDI-TOF/TOF-MS SELDI-TOF MS MALDI-TOF nLC-ESI-MS/MS
Further validation employed Yes (ELISA, western blot) No No Yes (Calgranulin A confirmed by western blot) Yes (ELISA and western blot) Yes (western blot)
Identified proteins ↑ haptoglobin,
↑ immunoglobulin kappa C,
↓ apolilipoprotein A-I,
↓ transthyretin
↑ α1-antitrypsin,
↓ transthyretin
↑ GRP75, mitochrondrial,
↑ Ubiquinol-cytochrome C reductase,
↑ Nidogen
↑ Calgranulin A,
↑ Calgranulin B,
↑ Calgranulin C
↑ α1-antitrypsin,
↑ myeoloperoxidase
↑ SFTP-A2,
↑ annexin-3,
↓ CLIC1,
↓ Calcyphosine