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Table 4 Peptide analytical parameters including within-run, between-run CV, LOD and LOQ

From: Quantification of angiotensin II-regulated proteins in urine of patients with polycystic and other chronic kidney diseases by selected reaction monitoring

Protein

Peptide sequence

Within-run CV (%)

Between-run CV (%)

LOD (fmol peptide)

LOD (fmol/µmol Cr)

LOQ (fmol peptide)

LOQ (fmol/µmolCr)

TSP-1

TIVTTLQDSIR

4.6

28

1.1

0.40

1.2

0.43

TSP-1

GGVNDFQGVLQNVR

4.7

32

1.3

0.43

2.3

0.75

GLUL

LVLCEVFK

8.4

16

0.17

0.06

1.1

0.39

RHOB

IQAYDYLECSAKa

7.6

24

0.1

0.04

0.1

0.04

BST1

GFFADYEIPNLQK

2.6

32

0.14

0.05

0.48

0.17

LYPLA1

LAGVTALSCWLPLRa

4.4

6

0.1

0.04

1.0

0.40

LAMB2

GSCYPATGDLLVGR

3.7

37

1.04

0.34

1.20

0.39

  1. Within-run and between-run CVs are presented as means of 11 urine samples for each peptide
  2. aLOD and LOQ could not be determined from statistical methods for this peptide. They were extrapolated from the calibration curve
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Clinical Proteomics

ISSN: 1559-0275