Fig. 1

a The chromatographic properties of CPB urine samples processed using 3, 10 or 30 kDa MWCO filters in the modified-FASP method. The total ion chromatogram of each sample is given. Note the peak spreading and altered retention times in the 3 and 10 kDa MWCO membrane processed samples. b The predicted molecular weight and pI distribution of proteins identified from the same urine sample FASP processed with 3, 10 or 30 kDa molecular weight cut-off membranes. The values beside each panel indicate the number of proteins with molecular weights less than 30 kDa and the total number of proteins identified in sample