Fig. 1

Target peptide selection and characterization. Top Sequence alignment of the 3 proteins (GP₁, sGP and ssGP) derived from the Ebola GP transcript showing the locations of target peptide candidates for use in the quantification of Ebola GP₁ (red dotted boxes) as well as the location of peptides rejected for the final assay (black boxes). All three protein products share sequence homology in the first 295 amino acids. Peptides identified in survey runs were evaluated for absence of post translational modifications, ionization efficiency and protein location. Bottom Schematic of fully processed GP_1,2 transmembrane protein, showing the location of the receptor binding site (RBS) and mucin-like domain (MLD) of GP₁, as well as the extracellular domain (ECD), transmembrane region (TM) and cytoplasmic tail (CT) of GP₂. GP₁ and GP₂ are disulfide linked to form the mature GP_1,2 complex