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Table 2 Evolution of approaches used to examine cardiac extracellular matrix [9, 10, 12, 20, 22]

From: Adapting extracellular matrix proteomics for clinical studies on cardiac remodeling post-myocardial infarction

Approach Strengths Limitations
1. Whole LV proteomics Routine protocol; fewest processing steps minimizes loss Selects for highly abundant proteins, which are mostly intracellular
2. Infarct region only proteomics Uses natural accumulation of ECM that occurs during scar formation Intracellular proteins still in high abundance
3. Decellularization proteomics Allows identification of a wide variety of proteins Difficult to reproducibly quantify
4. Glycoproteomics Provides reproducible quantification of glycosylated proteins Selects for glycosylated proteins
Molecular weight and pH information not provided