Table 2 Evolution of approaches used to examine cardiac extracellular matrix [9, 10, 12, 20, 22]
Approach | Strengths | Limitations |
|---|---|---|
1. Whole LV proteomics | Routine protocol; fewest processing steps minimizes loss | Selects for highly abundant proteins, which are mostly intracellular |
2. Infarct region only proteomics | Uses natural accumulation of ECM that occurs during scar formation | Intracellular proteins still in high abundance |
3. Decellularization proteomics | Allows identification of a wide variety of proteins | Difficult to reproducibly quantify |
4. Glycoproteomics | Provides reproducible quantification of glycosylated proteins | Selects for glycosylated proteins Molecular weight and pH information not provided |