Fig. 7
From: Mass spectrometry-based approaches to targeted quantitative proteomics in cardiovascular disease
HMGB1 structure. A linear diagram of high-mobility group box 1 protein (HMGB1) is shown, including the residues that constitute the A-box (pink), B-box (purple) and acidic tail (green). The proximal A-box and B-box of HMGB1 both contain putative nuclear-emigration signals, as identified by binding to the nuclear exportin chromosome-region maintenance 1. HMGB1 also contains 43 lysine residues, some of which are frequently acetylated in lipopolysaccharide-activated macrophages (shown in bold). These lysine residues are found within two nuclear-localization signals (indicated by dashed boxes): NLS1, which spans amino acids 28–44; and NLS2, which spans amino acids 179–185. Reprinted with permission from [72]