Fig. 3
From: Plasma degradome affected by variable storage of human blood
PROTOMAP workflow. The 30 min (Pool 1, n = 5) and 48 h samples (Pool 2, n = 5) were separated by 1-D SDS-PAGE and proteins visualised by Coomassie blue staining. The gel was subsequently divided into 22 bands per lane (representing one condition each). Each pool per band was cut to create n = 22 pieces per condition, proteins were subjected to in-solution trypsin digestion and analysed by LC-MS/MS. Raw MS data was analysed by PROTOMAP bioinformatics to generate peptographs [22]