Fig. 2

Flow chart of the experimental procedure and results of first and second screenings. A panel of 247 antibodies for 192 apoptosis-associated proteins was screened in surgically resected HCC and adjacent normal tissue samples. Single antibodies generated multiple protein bands by western blotting. Band intensity was compared between samples. a First screening using four sample pools such as group N (normal liver tissue), A (adjacent liver tissue), L (tumor tissue without recurrence), and S (tumor tissue with recurrence within 2 years of surgery). b Second screening for the selected 37 antibodies