Proteome analysis of excretory-secretory proteins of Entamoeba histolytica HM1:IMSS via LC–ESI–MS/MS and LC–MALDI–TOF/TOF

Table 1 Top 10 proteins of highest scores detected by LC–ESI–MS/MS

No	Accession	Name	−10lgP	Matched peptides	Sequence coverage (%)
Top ten proteins identified through LC–ESI MS/MS
1	EHI_150490	Aldehyde-alcohol dehydrogenase 2 putative	149.46	56	73
2	EHI_051060	Pyruvate:ferredoxin oxidoreductase	148.56	50	53
3	EHI_009530	Pyruvate phosphate dikinase	146.95	47	60
4	EHI_006980	Gal/GalNAc lectin subunit Igl1	135.22	35	39
5	EHI_077500	Galactose-specific adhesin 170kD subunit	134.04	36	34
6	EHI_030750	Hypothetical protein	133.28	39	50
7	EHI_133900	Galactose-inhibitable lectin 170 kDa subunit putative	133.14	37	35
8	EHI_044970	Malic enzyme putative	132.55	33	73
9	EHI_042370	Galactose-specific adhesin 170kD subunit putative	130.92	32	31
10	EHI_166920	Hypothetical protein	130.71	35	43

−10lgP A representation of p value, whereby the higher the −10lgP value is, the more significant the match is. A threshold score of above 20 signifies relatively high confidence
Matched peptides The number of high-confidence supporting peptide
Sequence coverage The percentage of protein sequence covered by peptide sequence

ISSN: 1559-0275