Fig. 1

Patterns of tumor invasion in vSCC. H&E (top panels) and picrosirius red (middle and bottom panels) staining of Push/LPC (left panels) and Inf/FMX (right panels) of vSCC. Tumor areas are indicated with a ‘#’ and stromal areas with a ‘*’. Picrosirius red staining is shown in bright field (middle panels) where collagen is stained red, and in polarized illumination (bottom panels) where larger collagen fibers are stained yellow-orange and thinner fibers, including reticular fibers, appear green. Photographed with ×20 objective