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Fig. 1 | Clinical Proteomics

Fig. 1

From: Quantitative proteomic analysis of pancreatic cyst fluid proteins associated with malignancy in intraductal papillary mucinous neoplasms

Fig. 1

Detailed experimental workflow. Pancreatic cyst fluid samples from nine individuals (3 LGD, 3 HGD, and 3 invasive IPMN) were included in this study. After centrifugation, pellets and debris were discarded, and the supernatant was collected for proteomic analysis. Proteins in each individual sample and pooled cyst fluid (comprised of equal portions of individual samples) were precipitated with cold acetone. Following FASP digestion, the pooled cyst fluids were fractionated using two types of high-pH fractionation methods. Every prepared sample was analyzed on a Q Exactive mass spectrometer

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