Fig. 2
From: Deep undepleted human serum proteome profiling toward biomarker discovery for Alzheimer’s disease
Basic pH RPLC elution profile and protein identification in individual fractions. a Extensive elution profile of pooled peptide mixture by basic pH RPLC. Peptides were eluted from a gradient buffer and monitored by UV absorption at 214 nm. Fractions were collected every minute. b Cumulative curve of total peptides and proteins by different combinations of fractions. For example, for the 90 alternative fractions, 24,083 peptides and 3890 proteins were identified