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Fig. 1 | Clinical Proteomics

Fig. 1

From: Comprehensive proteome and phosphoproteome profiling shows negligible influence of RNAlater on protein abundance and phosphorylation

Fig. 1

Experimental workflow. Tissues were cryopulverized to prepare frozen powder, of which one portion was treated directly with RNAlater while the other portion was stored at – 80 °C. After 24 h, samples were lysed for FASP digestion. Peptides were labeled with 6-plex TMT reagent, mixed, and then fractionated into 24 fractions for global proteome analysis or 12 fractions for phosphoproteome analysis. Each fraction was analyzed by an Orbitrap mass analyzer

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