Fig. 1From: Comprehensive proteome and phosphoproteome profiling shows negligible influence of RNAlater on protein abundance and phosphorylationExperimental workflow. Tissues were cryopulverized to prepare frozen powder, of which one portion was treated directly with RNAlater while the other portion was stored at – 80 °C. After 24 h, samples were lysed for FASP digestion. Peptides were labeled with 6-plex TMT reagent, mixed, and then fractionated into 24 fractions for global proteome analysis or 12 fractions for phosphoproteome analysis. Each fraction was analyzed by an Orbitrap mass analyzerBack to article page