Fig. 1
From: A proteome-wide immuno-mass spectrometric identification of serum autoantibodies
Methodology used to identify anti-CUZD1 serum autoantibodies with protein G magnetic beads. In the first step, serum is added to the beads, to capture serum antibodies, some of which are anti-CUZD1 autoantibodies (blue color). After washing, the beads are exposed to a protein mix, in this case pancreatic lysate or a more complex tissue lysate. In the third step, beads are washed and the captured proteins (including CUZD1; blue dots) are trypsin-digested before identification with tandem mass spectrometry. For more details see text