Fig. 1
From: Quantitative proteome analysis of Merkel cell carcinoma cell lines using SILAC
Experimental design of the study. MCC cells were cultured in medium supplemented with 13C6l-Lysine-2HCl (heavy) and HaCaT cells were cultured in medium supplemented with l-Lysine-2HCl (light). After tryptic digest of labelled proteins, peptides were analysed by mass spectrometry. A heat map was created to show cell line similarity. Specific proteins of each cell line (MCC13, MKL-1, MKL-2, PeTa and WaGa) were related in a Venn diagram. Furthermore, differentially enriched pathways were analysed. A heavy to light ratio of identified proteins was calculated and the up- and downregulation of MCC specific proteins was compared to the reference cell line HaCaT (Tables 1, 2)