Fig. 1

Laser capture microscopy of human lung blood vessels for mass spectrometry. a Blood vessels are identified by pentachrome stain (red arrows) and serial sections are used to laser microdissect blood vessels and adjacent alveoli. b A workflow of tissue preparation for mass spectrometry. Laser captured tissue is detergent treated, subjected to heat, resuspended with Urea, sheared using Covaris, and samples are later placed into a S-Trap Column for trypsin digest, followed by desalting prior to mass spectrometry loading