Fig. 4
From: Proteomic analysis of synovial fluid from rheumatic arthritis and spondyloarthritis patients
Localization of neutrophil proteins that correlate to cfDNA detected in SF. a Schematic drawing of a neutrophil and localisation of proteins correlating to cfDNA. Nucleus (blue) shows DNA and chromatin. Cytoplasmic granules are shown in pink. Annexin 1 is present in the granular membranes but translocated to the cytoplasmic membrane upon degranulation/NETs formation. Cytoplasm (light pink). b During NETosis proteins from all compartments can be released. In inflamed RA joint—both granular, cytoplasmic, nuclear proteins, Annexin 1 and cfDNA were detected in SF. Annexin 1 is an inhibitor of migration of neutrophil to inflammation. Metalloproteinase inhibitor 1 and Leukocyte elastase inhibitor were correlated to cfDNA. The origin of these inhibitors is unknown