Fig. 2From: Time dependent effect of cold ischemia on the phosphoproteome and protein kinase activity in fresh-frozen colorectal cancer tissue obtained from patientsMean protein tyrosine kinase (PTK) and serine/threonine kinase (STK) activity with CIT (peptide microarray). The mean signal intensity of all peptides at seven time points (T = 0, 30, 60, 90, 120, 150 and 180 min of CIT) for the tumors of all five patients (patient 1–5) for PTK and STK are represented (X-axis). The mean value for all samples is represented in a 2log scale (Y-axis). For each peptide values were the average of six datapoints. First, the median signal minus background values per array for all the technical replicates were calculated and then the average for the sample preparation replicates was calculated. Subsequently the mean of all peptides was calculatedBack to article page