Tear proteomic profile in three distinct ocular surface diseases: keratoconus, pterygium, and dry eye related to graft-versus-host disease

Table 2 Student’s t-test analysis of tear proteome from patients diagnosed with keratoconus versus control

Gene name	Protein	p-value	FDR 5%	FDR 1%	FWER	Control average LFQ log transformed	Keratoconus average LFQ log transformed	SE of difference
IGHV5-10–1; IGHV5-51	Immunoglobulin heavy variable 5–10-1; Immunoglobulin heavy variable 5–51	3.00E−03	N	N	N	21.8	23.2	0.3
PRR27	Proline-rich protein 27	3.10E−03	N	N	N	22.3	25.4	0.7
IGHM	Ig mu chain C region; Ig mu heavy chain disease protein	4.49E−03	N	N	N	26.0	27.8	0.4
HIST1H2BA§	Histone H2B type 1-A	4.68E−03	N	N	N	21.1	22.9	0.4
KRT13	Keratin, type I cytoskeletal 13	2.06E−02	N	N	N	25.5	27.4	0.7
IGHV3-23#	Immunoglobulin heavy variable 3–23	3.58E−02	N	N	N	24.5	25.5	0.4
DEFA1; DEFA3	Neutrophil defensin 1; HP 1–56; Neutrophil defensin 2; Neutrophil defensin 3; HP 3–56; Neutrophil defensin 2	3.69E−02	N	N	N	25.2	27.9	1.1

Proteins with increased abundance in keratoconus in comparison with controls are shown
FDR: false discovery rate. FEWR: family-wise error rate (Holm-Sidak method). Y: yes, still significant after multiple comparisons correction. N: no, not significant after multiple comparisons correction. SE: standard error. § histone cluster (HIST1H2BA; HIST1H2BK; HIST1H2BJ; HIST1H2BO; HIST1H2BB; H2BFS; HIST1H2BD; HIST1H2BC; HIST2H2BE; HIST2H2BF; HIST3H2BB; HIST1H2BH; HIST1H2BN; HIST1H2BM; HIST1H2BL). # immunoglobulin cluster (IGHV3-23; IGHV3-30; IGHV3-30–5)

ISSN: 1559-0275