Fig. 1
From: Global proteome profiling of human livers upon ischemia/reperfusion treatment
Flowchart detailing the experiments including the sample preparation, data acquisition, and data analysis performed in this study. The protein peptides digested by liver biopsies were collected from six patients with hepatic hemangioma underwent hepatectomy prior to Pringle maneuver (Control group) and 10 min after restoration of blood supply (Ischemia/reperfusion group) respectively, then these samples were subjected to TMT labeling, HPLC fractionation and biological analysis after LC–MS/MS, and verified by PRM, qPCR and western blotting