Table 1 List of significantly regulated proteins in ischemia/reperfusion group versus control group using LC–MS/MS approach

1

P00973

2′-5′-Oligoadenylate synthetase 1

OAS1

Activate of latent RNase L and induce viral RNA degradation

2

Q2TB90

Hexokinase domain containing 1

HKDC1

A member of the hexokinase involved in glucose metabolism

3

Q96AM1

MAS related GPR family member F

MRGPRF

Regulate nociceptor function and/or development

4

Q9UBS3

DnaJ (Hsp40) homolog

DNAJB9

Induced by ER stress and protect stressed cells from apoptosis

5

O00767

Stearoyl-CoA desaturase

SCD

An enzyme involved in fatty acid biosynthesis

6

Q9UNA1

Rho GTPase activating protein 26

ARHGAP26

A GTPase activating protein that binds to focal adhesion kinase

7

P20591

Interferon-inducible protein P78

MX1

A GTP-metabolizing protein involved in the cellular antiviral response

8

Q01581

HMG-CoA synthase

HMGCS1

Regulation of cholesterol biosynthesis

9

P05161

ISG15 ubiquitin-like modifier

ISG15

A ubiquitin-like protein upon activation by IFN-α and IFN-β

10

P37268

Squalene synthase

FDFT1

Catalyze the reaction to form squalene in cholesterol biosynthesis

11

Q14397

Glucokinase regulator

GCKR

A regulatory protein that inhibits glucokinase in liver

12

Q8N126

Cell adhesion molecule 3

CADM3

A calcium-independent cell–cell adhesion protein

13

Q9Y6K5

Interferon-induced protein 56

IFIT1

Inhibit expression of viral messenger RNAs

14

Q9UK22

F-box protein 2

FBXO2

Function in phosphorylation-dependent ubiquitination

15

Q9TQE0

MHC class II HLA-DR beta 1 chain

HLA-DRB1

Present peptides derived from extracellular proteins

16

O60488

Long-Chain Acyl -CoA synthetase 4

ACSL4

Play a key role in lipid biosynthesis and fatty acid degradation

17

O75604

Ubiquitin specific peptidase 2

USP2

A ubiquitin-specific protease required for TNF-α-induced NF-κB

18

Q16610

Extracellular matrix protein 1

ECM1

Involved in angiogenesis and tumor biology

19

Q9NR19

Acetyl-coenzyme a synthetase 2

ACSS2

Activation of acetate for use in lipid synthesis and energy generation

20

Q15800

Methylsterol monooxygenase 1

MSMO1

Localized to the ER membrane function in cholesterol biosynthesis

21

P11509

Cytochrome P450 family 2

CYP2A6

Involved in of cholesterol, steroids and other lipids

22

Q8TF05

Phosphatase 4 regulatory subunit 1

PPP4R1

Serine/threonine protein phosphatase 4 (PP4) to regulate HDAC3

23

P14555

Phospholipase A2 group IIA

PLA2G2A

Participate in phospholipid metabolism in biomembranes

24

P07355

Annexin A2

ANXA2

Members of this calcium-dependent phospholipid-binding protein family

25

O94760

Dimethylargininase-1

DDAH1

Plays a role in nitric oxide generation

26

Q9NYL2

MAPKKK20

MAP3K20

A member of MAPKKK family and play a role in cell cycle checkpoint

27

P60903

S100 calcium binding protein A10

S100A10

Involved in the regulation of cell cycle progression and differentiation

28

P02654

Apolipoprotein C1

APOC1

Play a central role in HDL and VLDL metabolism

29

Q15582

Transforming growth factor β

TGFBI

Modulate cell adhesion and serves as a ligand for several integrins

30

Q9H6E4

Coiled-coil domain containing 134

CCDC134

Inhibit ERK and JNK signal to suppress cell migration and invasion

31

P02750

Leucine rich alpha-2-glycoprotein 1

LRG1

Protein–protein interaction, and cell adhesion and development

32

P09917

Arachidonate 5-lipoxygenase

ALOX5

Synthesis of leukotrienes to mediate inflammatory and allergy

33

P14780

Matrix metallopeptidase 9

MMP9

Breakdown of extracellular matrix in tissue remodeling

34

P05164

Myeloperoxidase

MPO

Host defense system of polymorphonuclear leukocytes

35

P09601

Heme oxygenase 1

HMOX1

Cytoprotective effects against apoptosis

36

P30530

AXL receptor tyrosine kinase

AXL

Tyrosine kinase receptor involved in growth, migration, aggregation

37

Q9BYE9

Cadherin related family member 2

CDHR2

Calcium-dependent cell–cell adhesion molecules

38

Q9BRR9

Rho GTPase activating protein 9

ARHGAP9

A member of the Rho-GAP family of GTPase activating proteins

39

P55290

Cadherin 13

CDH13

Protects cells from apoptosis due to oxidative stress

40

P02776

Platelet factor 4

PF4

A member of the CXC chemokine family

41

P55008

Allograft inflammatory factor 1

AIF1

Play a role in macrophage activation and function

42

A0A075B6K5

Immunoglobulin lambda variable

IGLV3-9

Participates in the antigen recognition

43

Q15165

Paraoxonase 2

PON2

A cellular antioxidant, protecting cells from oxidative stress

44

Q9ULU4

Zinc finger MYND-type containing 8

ZMYND8

Down-regulation of diverse metastasis-associated genes

45

Q8TD30

Glutamic–alanine transaminase

GPT2

Catalyzes the reversible transamination between alanine and glutamate

46

P02763

Orosomucoid 1

ORM1

An acute-phase reactant

47

Q9H799

Ciliogenesis and planar polarity effector 1

C5orf42

Establishment of cell polarity required for directional cell migration

48

P09210

Glutathione S-transferase alpha 2

GSTA2

Glutathione S-transferase

49

P05413

Fatty acid binding protein 3

FABP3

Participate in intracellular metabolism of long-chain fatty acids

50

P80303

Nucleobindin 2

NUCB2

Have a role in calcium homeostasis

51

Q96JA1

Leucine-rich repeat protein LRIG1

LRIG1

A feedback negative regulator of signaling by receptor tyrosine kinases

52

Q5R3K3

Calcium homeostasis modulator 6

CALHM6

Calcium homeostasis

53

O00479

High mobility group nucleosomal binding domain 4

HMGN4

Reduce the compactness of the chromatin fiber in nucleosomes

54

Q14CZ8

Hepatocyte cell adhesion molecule

HEPACAM

Involved in cell motility and cell–matrix interactions

55

P30711

Glutathione S-transferase Theta 1

GSTT1

A member of glutathione s-transferase

56

O94885

SAM and SH3 domain containing 1

SASH1

A scaffold protein involved in the tlr4 signaling pathway

57

Q86VW2

GTP-rho-binding protein 2

RHPN2

A member of rho-gtpase binding proteins

58

P02741

C-reactive protein

CRP

Acute phase response to tissue injury, infection, or inflammatory stimuli

59

Q03013

Glutathione S-transferase Mu 4

GSTM4

A member of glutathione S-transferase

60

P09488

Glutathione S-transferase Mu 1

GSTM1

A member of glutathione S-transferase