Variable blood processing procedures contribute to plasma proteomic variability

Table 3 Top ten proteins with the most statistically significant associations (the lowest BH-FDR adjusted p-values) for the main effect of the anticoagulant type in multi-donor blood processing study

Accession	Description	logFC	AveExpr	BH-FDR
P27169	PON1: serum paraoxonase/arylesterase 1	− 2.12	11.09	< 10^–5
P00915	CA1: carbonic anhydrase 1	− 1.19	10.14	< 10^–5
Q99969	RARRES2: retinoic acid receptor responder protein 2	1.88	7.31	< 10^–5
P80108	GPLD1: phosphatidylinositol-glycan-specific phospholipase D	− 5.22	8.62	< 10^–5
P12259	F5: coagulation factor V	0.39	11.62	0.00046
P02788	LTF: lactotransferrin	2.00	7.29	0.00046
P02747	C1QC: complement C1q subcomponent subunit C	− 0.99	9.83	0.00051
P00918	CA2: carbonic anhydrase 2	− 1.34	8.29	0.00085
P10124	SRGN: serglycin	1.58	6.99	0.00122
P02746	C1QB: Complement C1q subcomponent subunit B	− 1.51	10.59	0.00122

Negative values represent a decrease (on log base 2 scale of fold change) in protein levels (at zero hours, at room temperature) in heparin tubes compared to EDTA tubes. Column “AveExpr” represents average expression level of each protein on the scale of log base 2 CPM (as further explained in Additional file 1: Section S1)

ISSN: 1559-0275