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Table 1 List of gene names and primer sequences used to conduct qPCR

From: Integrative omics reveals subtle molecular perturbations following ischemic conditioning in a porcine kidney transplant model

Oligo name

Forward sequence (5′–3′)

Reverse sequence (5′–3′)

Scale (µmole)

PDZD3

CCCTGTAAGTGCCCTGCTAT

CTCCAGGGATCAGAAGGATCG

0.025 µmole

LTB4R

TGGTACTTCCTCTCTGGCTGA

CCATTGCAAGGACAGGCTTT

0.025 µmole

RASL10A

TTGCATTTGGGGTAAACCTGGAA

GCCACTCCTCCAAGCTTAATTC

0.025 µmole

SLC16A3

AGGTAACCTGAGACCTGGCT

TGGTTCCGCGTCCCTGG

0.025 µmole

IL1B

CCAATTCAGGGACCCTACCC

GTTTTGGGTGCAGCACTTCAT

0.025 µmole

Beta actin

TGTCATGGACTCTGGGGATG

GGGCAGCTCGTAGCTCTTCT

0.025 µmole

  1. A total of five genes were selected for qPCR validation of transcriptomics results, with beta actin serving as a control
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Clinical Proteomics

ISSN: 1559-0275