Fig. 4
From: Systematic evaluation and optimization of protein extraction parameters in diagnostic FFPE specimens
Temperature effects in heat-induced antigen retrieval. A Heatmaps showing the relative quantities and concentrations; distribution arrays were normed to the interval [0,1] with 0 = minimum (yellow) and 1 = maximum value (dark red) and averaged for n = 2 samples (n = 1 for constant 2 h, blot reproduced once); combined marker quantity averaged beta-Integrin, ATP5B and HPRT–EpCAM was not included due to much lower expression levels; B Quantitative differences of subplot A as overlay of sample-specific distributions (n = 2); samples were normed to their parameter-specific mean to enable comparison between replicates chosen to cover different levels of overall protein content; visualization by tenfold linear interpolation to improve visibility of color coding (0 = yellow, max = dark red)