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Fig. 5 | Clinical Proteomics

Fig. 5

From: Optimized sample preparation and data analysis for TMT proteomic analysis of cerebrospinal fluid applied to the identification of Alzheimer’s disease biomarkers

Fig. 5

Measured TMT ratios of spiked pepmix proteins in MS2 and MS3 mode. Pepmix TMT ratios at each specified spike-in amount were evaluated separately for all three proteins P00489 (GPb, green), P00330 (ADH, red) and P00924 (enolase, blue) in MS2 (A) and MS3 (B) mode. Error bars indicate standard deviation for triplicate measurements. The dotted trendline represents the theoretically expected linear trend of TMT ratios. C Summarized mean TMT ratios of P00489 and P00330 plotted against their corresponding spike-in amount (unnormalized data). TMT ratios measured in MS2 mode are colored in red and TMT ratios measured in MS3 mode are depicted in blue

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Clinical Proteomics

ISSN: 1559-0275