Table 1 Parameters evaluating the completeness of the TMT labeling reaction at 0.5 mg, 0.25 mg, and 0.1 mg per 50 µL CSF sample
Amount TMT/50 µL CSF [mg] | Labeling efficiency [%] | Median peptide CV [%] | Median protein CV [%] | Median log2(MRII) |
|---|---|---|---|---|
0.5 | 91.42 | 43.9 | 36.9 | 16.2 |
0.25 | 87.93 | 47.5 | 36.1 | 14.5 |
0.1 | 79.98 | 45.5 | 34.6 | 15.7 |