Fig. 4

Validation of HRG and C4B expression in enlarged RSA and Control gruop patients. A ELISA validation of HRG expression in 41 RSA and 41 Control group patients. B ROC analysis of HRG. C ELISA validation of C4B expression in 36 RSA and 36 Control group patients. C, D Receiver operating characteristic (ROC) curves of HRG and C4B respectively. The test variable was protein expression level and the classification variable was the outcome of the current IVF cycle. E The ROC curve of HRG and C4B combined, the test variable was the predicted probability calculated by logistic regression of C4B and HRG in the same FF sample. The blue solid line represents the ROC curve. The diagonal line (red dashed line) from [0, 0] to [1, 1] is the line of equivalence. The p-value and area under the curve (AUC) was calculated and shown in the lower right corner of each graph