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Fig. 4 | Clinical Proteomics

Fig. 4

From: Phospho-heavy-labeled-spiketide FAIMS stepped-CV DDA (pHASED) provides real-time phosphoproteomics data to aid in cancer drug selection

Fig. 4

Analysis of phosphoproteome and phosphopeptide characteristics identified using single-shot LFQ compared with pHASED. A Overlap of phosphoprotein accessions comparing single-shot LFQ and pHASED experiments. B Number of single and multi-phosphorylated peptides identified in each experiment comparing single-shot LFQ and pHASED (statistical significance was determined via ordinary two-way ANOVA). C Number of phosphopeptides per protein identified in single-shot LFQ and pHASED experiments (unpaired t-test). D Number of high confidence phosphorylated master protein kinases identified in each single-shot LFQ and pHASED experiment (unpaired t-test). Statistical threshold: *p < 0.05, **p < 0.01 and ***p < 0.001

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Clinical Proteomics

ISSN: 1559-0275