Mitochondria and cytochrome components released into the plasma of severe COVID-19 and ICU acute respiratory distress syndrome patients

Table 1 A comparison of corrected mean observation frequency per gene symbol for common serum proteins and acute phase response proteins in EDTA plasma across COVID-19, ICU-ARDS and Normal Human Plasma (NHP)

Gene_symbol	COVID-19			COVID-19			COVID-19			ICU-ARDS
	X!TANDEM			SEQUEST			SEQUEST			X!TANDEM
	TRYP/STYP	Delta	χ2	TRYP	Delta	χ2	STYP	Delta	χ2	TRYP/STYP	Delta	χ2
AANAT	2	-3	2	8	5	6	48	11	3	1	-4	3
AAT	48	-77	47	155	-228	135	13	5	3	148	23	4
ABO	0	0	0	22	7	3	33	10	4	0	0	0
ACHE	5	3	3	65	18	7	105	48	40*	5	3	3
ALB	6022	398	28*	105,303	14,524	2324*	19,572	2942	520*	5843	219	9*
APCS	311	279	2359*	640	472	1318*	134	65	60*	174	142	611*
APP	2	0	0	26	11	8	39	11	4	1	-1	0
AZGP1	451	363	1481*	944	742	2712*	89	57	98 *	153	65	47*
B2M	57	56	1568*	268	233	1508*	10	0	0	48	47	1105*
CRP	151	151	22,801*	618	611	46,665*	180	161	1296*	234	234	54,756*
FGA	236	-522	359	636	-1141	732	410	-569	330	528	-230	70
HBA1	128	64	63*	336	192	254*	72	54	153*	149	85	111*
HP	896	234	83*	5613	2910	3132*	752	403	464*	1249	587	520*
HPR	574	183	85*	3203	1841	2487*	1302	757	1050*	788	397	402*
HPX	217	-235	122	863	-1186	686	226	-217	106	538	86	16*
HuSAA1g	0	0	0	3	1	0	11	9	27*	0	0	0
ITIH4	24	-119	98	190	-333	212	134	-70	24	75	-68	32
KLKB1	0	0	0	59	27	22*				0	0	0
LU	0	0	0	90	40	31*	95	21	6	0	0	0
ORM1	1642	1363	6635*	12,752	11,529	108,593*	2525	2294	22,683*	1278	999	3564*
ORM2	894	732	3287*	9138	8350	88,368*	2763	2479	21,563*	630	468	1344*
PON1	1	-7	5	14	-32	22	14	-8	3	2	-6	4
PON2	3	3	9	35	-3	0	50	-7	1	2	2	4
PON3	1	1	1	22	-3	0	16	-9	3	5	5	25*
RBP4	270	177	333*	1570	1093	2499*	331	147	117 *	183	90	86*
RhCE	0	0	0	16	11	20*	17	14	49*	0	0	0
RHD	0	0	0	26	-16	6	7	2	1	0	0	0
RhDTI	0	0	0	37	-27	11	0	0	0	0	0	0
RhIVb(J)	0	0	0	32	-30	14	0	0	0	0	0	0
S100A10	9	4	3	97	26	9*	149	-3	0	5	0	0
S100A14	0	-2	1	85	55	98*	34	-3	0	0	-2	1
S100A7A	1	-2	1	46	22	19*	50	13	4	0	-3	2
S100A8	5	3	3	95	53	65*	29	14	12*	37	35	408*
S100A9	32	30	300*	78	76	1925*	18	11	15*	91	89	2640*
SAA1	326	312	6490*	895	869	27,969*	0	0	0	545	531	18,797*
SAA2	150	141	1988*	525	508	14,337*	0	0	0	290	281	7896*
SAA2-SAA4	136	-16	2	506	306	466*	0	0	0	284	132	114*
SAA4	3	-139	135	23	-166	145	0	0	0	11	-131	120
SAAL1	0	0	0	33	-13	4	23	-9	2	0	0	0
TF	323	-494	298	1116	-2446	1679	180	-124	50	860	43	2
TFRC	1	-2	1	13	1	0	34	14	9	2	-1	0

Gene_symbol	ICU-ARDS			ICU-ARDS			NHP			OIT
	SEQUEST			SEQUEST			X!TANDEM	SEQUEST		X!TANDEM	SEQUEST
	TRYP	Delta	χ2	STYP	Delta	χ2	TRYP/STYP	TRYP	STYP	TRYP/STYP	TRYP	STYP
AANAT	8	5	6	27	-10	3	5	3	37	0	2	12
AAT	510	127	42*	24	16	28*	125	383	8	41	105	4
ABO	25	10	6	30	7	2	0	15	23	0	2	15
ACHE	41	-6	1	59	2	0	2	47	57	2	5	5
ALB	113,793	23,014	5834*	21,879	5249	1657*	5624	90,779	16,630	1307	11,487	2291
APCS	387	219	284*	54	-15	3	32	168	69	137	236	15
APP	16	1	0	23	-5	1	2	15	28	0	12	11
AZGP1	340	138	94*	47	15	7	88	202	32	175	348	25
B2M	190	155	667*	12	2	0	1	35	10	9	23	1
CRP	864	857	91,806*	300	281	3948*	0	7	19	19	39	15
FGA	1574	-203	23*	1133	154	24*	758	1777	979	255	382	102
HBA1	384	240	397*	127	109	625*	64	144	18	54	103	42
HP	8743	6040	13,492*	1089	740	1565*	662	2703	349	262	794	105
HPR	4700	3338	8175*	1833	1288	3038*	391	1362	545	99	528	209
HPX	2275	226	25*	508	65	10*	452	2049	443	181	629	68
HuSAA1g	5	3	3	9	7	16*	0	2	2	0	0	3
ITIH4	281	-242	112	161	-43	9	143	523	204	247	433	26
KLKB1	52	20	12*				0	32		0	39	0
LU	98	48	45*	126	52	36*	0	50	74	0	13	1
ORM1	10,140	8917	64,962*	2298	2067	18,416*	279	1223	231	355	1589	173
ORM2	7269	6481	53,236*	2500	2216	17,230*	162	788	284	261	1019	264
PON1	15	-31	20	8	-14	9	8	46	22	28	35	16
PON2	20	-18	8	47	-10	2	0	38	57	0	6	5
PON3	17	-8	2	18	-7	2	0	25	25	0	8	1
RBP4	772	295	182*	213	29	5	93	477	184	86	564	100
RhCE	16	11	20*	9	6	9	0	5	3	0	0	2
RHD	30	-12	3	6	1	0	0	42	5	0	1	2
RhDTI	44	-20	6	0	0	0	0	64	0	0	1	0
RhIVb(J)	31	-31	15	0	0	0	0	62	0	0	1	0
S100A10	62	-9	1	93	-59	23	5	71	152	0	20	12
S100A14	49	19	12*	24	-13	4	2	30	37	0	0	1
S100A7A	51	27	29*	35	-2	0	3	24	37	0	18	39
S100A8	226	184	787*	81	66	272*	2	42	15	9	38	10
S100A9	246	244	19,845*	36	29	105*	2	2	7	25	62	16
SAA1	1451	1425	75,208*	0	0	0	14	26	0	77	99	0
SAA2	880	863	41,376*	0	0	0	9	17	0	28	39	0
SAA2-SAA4	876	676	2274*	0	0	0	152	200	0	51	66	0
SAA4	40	-149	117	0	0	0	142	189	0	31	41	0
SAAL1	33	-13	4	24	-8	2	0	46	32	0	5	1
TF	2968	-594	99	431	127	53	817	3562	304	246	1053	126
TFRC	17	5	2	38	18	15	3	12	20	0	3	3

The SQL SERVER accession descriptions that contained “sera” or “serum” or “sero” or “plasma” not “membrane” were queried. The observation frequency was computed after correcting for the number of MS/MS spectra in each treatment with precursor intensity ≥ E3 detector counts. The spectra were fit to tryptic peptides (TRYP) or optionally phospho/tryptic (STYP) peptides by the X!TANDEM algorithm. The peptides were fit to tryptic peptides (TRYP) or obligate phosphotryptic peptides by the SEQUEST algorithm. The difference (Delta) and Chi Square value χ2 with respect to NHP are shown. The asterisk * indicates a significant increase in observation frequency by the Chi Square test in the COVID-19 or ICU-ARDS plasma compared to the Normal Human Plasma (NHP) with χ2 ≥ 9, p ≤ 0.01

ISSN: 1559-0275