Fig. 1
From: Changes of serum IgG glycosylation patterns in rheumatoid arthritis
The methodology for the identification of lectin–glycan interaction was illustrated from left to right. Immobilized on a glass surface, lectins can be probed with unlabeled glycoprotein, which could be detected by fluorescently labeled antibody. The signals obtained from the lectin microarrays are subjected to statistical analysis. Based on the known location of the 56 lectins on the microarray (Fig. 1), identification of the interacting glycan can be done effectively