Fig. 1

Study design of the two-stage workflow. RMS rhabdomyosarcoma, HC healthy control, RN newly diagnosed RMS patients at the time points of admission, RS RMS patients underwent surgery at the time points of admission, T0 Timepoint of the first day of admission, T1 Timepoint after 4 cycles of chemotherapy, DIA data-independent acquisition, PRM parallel reaction monitoring analysis, DP differential proteins, ROC receiver operating characteristic curve