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Fig. 1 | Clinical Proteomics

Fig. 1

From: An optimized workflow for MS-based quantitative proteomics of challenging clinical bronchoalveolar lavage fluid (BALF) samples

Fig. 1

Key steps of BALF processing workflow for quantitative LC–MS/MS analysis. The workflow includes value-added collection of endogenous peptides for analysis (if desired), along with immunoaffinity-based depletion of the 14 most abundant plasma proteins, as well as protein trapping for concentration, clean-up, and tryptic digestion. The resulting peptides are compatible with isobaric peptide labeling or label-free analysis by LC–MS/MS

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Clinical Proteomics

ISSN: 1559-0275