Table 3 Amenability of the workflow to material-limited samples and the effects of microfractionation on sampling depth. Averages for peptides and proteins identified across three 16-plex TMT groups are shown
No fractionation | With microfractionation | ||
|---|---|---|---|
Peptides identified (1% FDR) | Protein groups (1% FDR) | Peptides identified (1% FDR) | Protein groups (1% FDR) |
4770 ± 1726 | 524 ± 77 | 9902 ± 933 | 1127 ± 57 |