Fig. 1

Infographic of plasma proteins identified by label-free quantitative proteomics in flow-through (A) and elute (D) fractions of pooled plasma from healthy controls, non-metastatic CRC patients and metastatic CRC patients separated by MARS-14 immunochromatography. Volcano plots of the protein expression levels in non-metastatic and metastatic CRC patients in comparison to those of the healthy controls in the flow-through (B and C) and elute (E and F) fractions, respectively. The x-axis represents log₂ fold changes of proteins and the y-axis represents -log₁₀p-value. The red and green dots indicate proteins with significantly different expression identified by log₂ fold change > 0.58 and < -0.58 (> 1.5-fold differential expression), and -log₁₀p-value greater than 1.3 (p-value < 0.05), respectively. The black dots indicate proteins which were not significantly altered between CRC patients and healthy control groups.