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Fig. 2 | Clinical Proteomics

Fig. 2

From: Label-free quantitative proteomics reveals aberrant expression levels of LRG, C9, FN, A1AT and AGP1 in the plasma of patients with colorectal cancer

Fig. 2

The protein patterns and expression levels of LRG, S100A8, C9, FN, AGP1, and A1AT in crude plasma, MARS-14 flow-though (FT) and MARS-14 elute (EL) fractions of the pooled samples of healthy controls (HC), patients with non-metastatic CRC (NM) and patients with metastatic CRC (M). (A) Stain-free imaging of the gel displayed the pattern of total proteins from three groups (HC, NM, and M). Crude plasma (30 µg), flow-through (5 µg) and elute (5 µg) fractions of three groups were separated on 10% TGX stain-free FastCast. Total proteins were visualized by a stain-free imaging system. (B) Immunoblots of LRG, S100A8, C9, FN, AGP1, and A1AT. Values below immunoblots denote the ratio of each protein band intensity normalized by its total protein loading and compared to those of the healthy control.

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Clinical Proteomics

ISSN: 1559-0275