Glial cell proteome using targeted quantitative methods for potential multi-diagnostic biomarkers

Kang, Narae; Oh, Hyun Jeong; Hong, Ji Hye; Moon, Hyo Eun; Kim, Yona; Lee, Hyeon-Jeong; Min, Hophil; Park, Hyeonji; Lee, Sang Hun; Paek, Sun Ha; Jin, Jonghwa

doi:10.1186/s12014-023-09432-x

Clinical Proteomics

Table 2 Selected 20 proteins and MRM transition

From: Glial cell proteome using targeted quantitative methods for potential multi-diagnostic biomarkers

No	Gene name	Peptide sequence^a	Q1^b (Da)	Q3^c (Da)	Charge^d	MRM (T-test)^e			MRM (AUC)^f
No	Gene name	Peptide sequence^a	Q1^b (Da)	Q3^c (Da)	Charge^d	(C vs G2)	(C vs G4)	(C vs G3 and G4)	(C vs G2)	(C vs G4)	(C vs G3 and G4)
1	ATP2B4	Light EGDFGCTVMELR Heavy EGDFGCTVMEL[13C-15N-R]	Light 678.8 Heavy 683.804	Light 908.433 Heavy 918.441	2 +	0.524129	0.464897	0.87613	0.500	0.513	0.441
2	ATP5ME	Light ELAEDDSILK Heavy ELAEDDSIL[13C-15N-K]	Light 566.79 Heavy 570.797	Light 690.367 Heavy 698.381	2 +	0.158475	0.407572	0.090901	0.340	0.387	0.293
3	CCT3	Light AVAQALEVIPR Heavy AVAQALEVIP[13C-15N-R]	Light 583.848 Heavy 88.852	Light 797.488 Heavy 807.496	2 +	0.4499	0.004443	0.006446	0.600	0.867	0.800
4	DNMT1	Light VARPLPAEEPER Heavy VARPLPAEEPE[13C-15N-R]	Light 682.37 Heavy 87.374	Light 1037.526 Heavy 1047.534	2 +	0.640442	0.069085	0.243172	0.650	0.853	0.689
5	FKBP2	Light LVIPSELGYGER Heavy LVIPSELGYGE[13C-15N-R]	Light 666.862 Heavy 671.866	Light 1007.479 Heavy 1017.488	2 +	0.282686	0.511163	0.46159	0.570	0.587	0.578
6	GLRX5	Light DYAAYNVLDDPELR Heavy DYAAYNVLDDPEL[13C-15N-R]	Light 827.391 Heavy 832.396	Light 1070.548 Heavy1080.556	2 +	0.523981	0.800963	0.171209	0.370	0.480	0.304
7	IDH3A	Light WMIPSEAK Heavy WMIPSEA[13C-15N-K]	Light 481.244 Heavy 485.251	Light 531.277 Heavy 539.292	2 +	0.616804	0.060986	0.294098	0.420	0.687	0.556
8	JAM2	Light ATTMSENDFK Heavy ATTMSENDF[13C-15N-K]	Light 572.253 Heavy 576.26	Light 739.326 Heavy 747.34	2 +	0.336735	0.072686	0.171878	0.580	0.707	0.644
9	LDHA	Light VTLTSEEEAR Heavy VTLTSEEEA[13C-15N-R]	Light 567.786 Heavy 572.79	Light 821.364 Heavy 831.372	2 +	0.098785	0.562608	0.087983	0.710	0.420	0.296
10	PCMT1	Light: VFEVMLATDR Heavy: VFEVMLATD[13C-15N-R]	Light 590.805 Heavy 595.809	Light 934.466 Heavy 944.474	2 +	0.580865	0.004685	0.317842	0.510	0.833	0.600
11	PLEKHG3	Light SIVEDYLLK Heavy SIVEDYLL[13C-15N-K]	Light 540.303 Heavy 544.31	Light 879.482 Heavy 887.496	2 +	0.236567	0.025892	0.440049	0.590	0.227	0.407
12	PRDX6	Light LPFPIIDDR Heavy LPFPIIDD[13C-15N-R]	Light 543.303 Heavy548.307	Light 728.394 Heavy 738.402	2 +	0.187465	0.263135	0.450441	0.310	0.593	0.411
13	SLC44A2	Light YLTYLNAR Heavy YLTYLNA[13C-15N-R]	Light 507.274 Heavy 512.279	Light 737.394 Heavy 747.402	2 +	0.552204	0.106011	0.376704	0.570	0.680	0.659
14	TACC3	Light AQAEALALQASLR Heavy AQAEALALQASL[13C-15N-R]	Light 671.378 Heavy 676.382	Light 1142.653 Heavy 1152.661	2 +	0.452203	0.183185	0.402999	0.450	0.707	0.637
15	TINAGL1	Light ITGWGEETLPDGR Heavy ITGWGEETLPDG[13C-15N-R]	Light 715.849 Heavy 720.853	Light 444.22 Heavy 454.228	2 +	0.232988	0.935397	0.507493	0.540	0.267	0.219
16	TKT	Light AYGQALAK Heavy AYGQALA[13C-15N-K]	Light 411.229 Heavy 415.237	Light 587.351 Heavy 595.365	2 +	0.522284	0.000576	0.293373	0.390	0.873	0.644
17	TOMM34	Light AAGNESFR Heavy AAGNESF[13C-15N-R]	Light 426.204 Heavy 431.208	Light 709.326 Heavy 719.335	2 +	0.044977	0.013853	0.019581	0.840	0.880	0.826
18	UACA	Light YAPIVSFEECER Heavy YAPIVSFEECE[13C-15N-R]	Light 750.345 Heavy 755.35	Light 956.378 Heavy 966.386	2 +	0.4204	0.48008	0.06091	0.620	0.433	0.281
19	UBA1	Light GLGVEIAK Heavy GLGVEIA[13C-15N-K]	Light 393.74 Heavy 397.747	Light 616.366 Heavy 624.381	2 +	0.944065	0.018036	0.153785	0.460	0.747	0.600
20	YWHAE	Light YDEMVESMK Heavy YDEMVESM[13C-15N-K]	Light 566.238 Heavy 570.246	Light 853.379 Heavy 861.394	2 +	0.44896	0.218744	0.207092	0.390	0.620	0.607

^aSequence represents the sequence of proteotypic peptide for target protein
^b–cQ1 and Q3 (m/z) represent the Q1 and Q3 transitions for proteotypic peptide, respectively
^dCharge represents the charge state of precursor ion
^eT-test and
^fAUC represent the p-value and area under the curve values in MRM, where C, G2, G3, and G4 are control, grade 2, grade 3, and grade4, respectively

Back to article page

ISSN: 1559-0275

Contact us

Submission enquiries: journalsubmissions@springernature.com