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Table 2 The 23 proteins and their corresponding light- and heavy-isotope labeled peptides used for the PRM assay

From: Quantification of putative ovarian cancer serum protein biomarkers using a multiplexed targeted mass spectrometry assay

UniProt accession number

Protein name

Synthetic Light (Lys0 or Arg0) and Heavy (Lys8 or Arg10) peptides

References

sp|Q8WXI7|MUC16_HUMAN

Mucin-16 (CA125)

LTNDIEELGPYTLDR*, VAIYEEFLR*, VISPVVTSSSIR*, STISSLGTPSISTK*, ELGPYTLDR*

[70, 71]

sp|P22223|CADH3_HUMAN

Cadherin-3

FTQDTFR*, EPLLLPEDDTR*, ETGWLLLNKPLDR*, STGTISVISSGLDR*, LTVTDLDAPNSPAWR*

[72]

sp|P02771|FETA_HUMAN

Alpha-fetoprotein

DFNQFSSGEK*, NIFLASFVHEYSR*

[73, 74]

sp|P09038|FGF2_HUMAN

Fibroblast growth factor 2

LESNNYNTYR*, YTSWYVALK*

[75]

sp|P15328|FOLR1_HUMAN

Folate receptor alpha

DVSYLYR*, GWNWTSGFNK*, VLNVPLCK*

[76]

sp|P19883|FST_HUMAN

Follistatin

LSTSWTEEDVNDNTLFK*, SIGLAYEGK*

[77]

sp|Q99988|GDF15_HUMAN

Growth/differentiation factor 15

YEDLLTR*, AALPEGLPEASR*

[78, 79]

sp|P08833|IBP1_HUMAN

Insulin-like growth factor-binding protein 1

IPGSPEIR*, ALPGEQQPLHALTR*

[80]

sp|P18065|IBP2_HUMAN

Insulin-like growth factor-binding protein 2

LIQGAPTIR*, LEGEACGVYTPR*

[81]

sp|P05231|IL6_HUMAN

Interleukin-6

VLIQFLQK*, NLDAITTPDPTTNASLLTK*, YILDGISALR*, EALAENNLNLPK*

[78, 82]

sp|Q92876|KLK6_HUMAN

Kallikrein-6

LSELIQPLPLER*, PGVYTNVCR*

[78, 83, 84]

sp|Q9UBX7|KLK11_HUMAN

Kallikrein-11

CANITIIEHQK*, LLCGATLIAPR*

[78, 85]

sp|P08727|K1C19_HUMAN

Keratin, type I cytoskeletal 19

AALEDTLAETEAR*, ALEAANGELEVK*, DAEAWFTSR*, ILGATIENSR*, DYSHYYTTIQDLR*

[86]

sp|Q13421|MSLN_HUMAN

Mesothelin

IQSFLGGAPTEDLK*, GLLPVLGQPIIR*, ANVDLLPR*, TDAVLPLTVAEVQK*

[87]

sp|P15941|MUC1_HUMAN

Mucin-1

NYGQLDIFPAR*

[88, 89]

sp|Q96NY8|NECT4_HUMAN

Nectin-4

NPLDGSVLLR*, LDGPLPSGVR*, GDSGEQVGQVAWAR*, VSTFPAGSFQAR*

[90]

sp|P10451|OSTP_HUMAN

Osteopontin

GDSVVYGLR*, AIPVAQDLNAPSDWDSR*, YPDAVATWLNPDPSQK*

[91]

sp|P01236|PRL_HUMAN

Prolactin

LLEGMELIVSQVHPETK*, LSAYYNLLHCLR*

[92]

sp|Q16651|PRSS8_HUMAN

Prostasin

LGAHQLDSYSEDAK*, PLQQLEVPLISR*

[78, 93]

sp|P04278|SHBG_HUMAN

Sex hormone-binding globulin

IALGGLLFPASNLR*, QAEISASAPTSLR*, LDVDQALNR*

[68]

sp|P01033|TIMP1_HUMAN

TIMP Metalloproteinase inhibitor 1

GFQALGDAADIR*, SEEFLIAGK*, EPGLCTWQSLR*

[94]

sp|P25445|TNR6_HUMAN

Tumor necrosis factor receptor superfamily member 6

DITSDSENSNFR*

[95, 96]

sp|P35968|VGFR2_HUMAN

Vascular endothelial growth factor receptor 2

TFEDIPLEEPEVK*, DYVGAIPVDLK*, FLSTLTIDGVTR*

[78, 83, 97, 98]

  1. *Chemically synthesized 13C/15N-labeled peptides are used as internal standards; labeled amino acids are indicated in bold, and these peptides are labeled on the C-terminal Lys as K8 (13C6, 15N2) or C-terminal Arg as R10 (13C6, 15N4)
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Clinical Proteomics

ISSN: 1559-0275