Fig. 2

Linearity and binding efficiency of KiP. A Schematic for KiP with different input amounts and serial depletion. KiP was performed with increasing amounts of 7REF lysate. For the depletion experiments, KiP was repeated twice with the supernatant from 50 to 100 µg KiP experiments. B Number of kinases identified and kinase abundance from different input experiments. Kinase numbers are plotted in green and abundance is plotted in blue. C Number of kinases identified and kinase abundance from depletion experiments. Kinase numbers are plotted in green and abundance is plotted in blue