Fig. 4

KiP classifies 16 WHIM PDX tumors according to their intrinsic subtype. Kinases from 16 breast cancer xenografts were enriched by KiP, and druggable kinases and subtype-specific kinases were quantified by PRM. 50 μg of protein from PDX tumors was used, and all experiments were performed in duplicates. Clustering analysis of kinases distinguishes basal subtype samples from luminal subtype samples, and claudin-low samples are separated from all other samples. A basal specific kinase, EGFR is enriched in basal PDXs, and luminal specific kinases, RET and IGF1R, are enriched in luminal PDXs. KiP is able to capture most of subtype-specific kinases identified in previous RNA-sequencing or proteome profiling studies [24]