Fig. 8

Representative case (343VW) depicting variable molecular expression by LMD sampling level. Representative micrographs of H&E-stained tissue sections mounted on glass slides from the top of each sampling level. The tissue section number is notated in the bottom right corner. The scale bar in the bottom left corner of each micrograph indicates a length of 5 mm. The median tumor cellularity (%), as determined by manual pathology review, per level with relative standard deviation (%CV) is shown (middle table). The proteomic abundances of representative tumor/epithelial (CDH1 and KRT19) and stroma (FAP and VCAN) markers, and xCell cell type enrichment scores for epithelial cells and fibroblasts [21] are shown in the heatmap