Fig. 1
From: Simultaneous targeted and discovery-driven clinical proteotyping using hybrid-PRM/DIA
The hybrid-PRM/DIA acquisition scheme. Compared to conventional Parallel Reaction Monitoring (PRM) and Data-Independent Acquisition (DIA) methods, the hybrid-PRM/DIA approach utilizes rapid and simultaneous multiplexing of PRM MS/MS scans (MSxPRM) triggered by the detection of isotope-labeled heavy reference peptides. Successful detection of the isotope-labeled peptide triggers the high-quality measurement of the corresponding endogenous counter-peptide multiplexed with the isotope-labeled peptide by MSxPRM MS/MS scans. These scans are acquired with a narrower isolation window and maximized ion injection time for each species resulting in a higher sensitivity. Hybrid-PRM/DIA provides a data matrix with no missing data points of clinically relevant markers (heatmap gray: missing value; heatmap white: below LOD)