Fig. 3
From: Simultaneous targeted and discovery-driven clinical proteotyping using hybrid-PRM/DIA
Benchmarking of DIA performance in hybrid-PRM/DIA with an increasing number of triggered MSxPRM scans. A Protein groups identified through DIA in hybrid-PRM/DIA with a predefined list of TAA target peptides (179, 120, and 60 peptides) compared to standard DIA. The HeLa digest was spiked with a mixture of crude TAA heavy triggering peptides and TAA light peptides, both at an amount of 100 fmol per injection. The lower panel of Fig. A shows the median CV values for all proteins quantified in DIA and hybrid-PRM/DIA. B Identified peptides, identified peptides with CVs below 20% or 10% respectively are shown for the different numbers of hybrid-PRM/DIA peptide targets in MSxPRM mode