Fig. 2

Differentially expressed inflammatory proteins between healthy young and healthy elderly individuals. (A) Volcano map of differentially expressed proteins between healthy young and healthy elderly individuals. (B) Heatmap shows the top 30 differentially expressed proteins (p < 0.05) in healthy young individuals and healthy elderly individuals. (C, D The enrichment analysis results of GO and KEGG pathways are presented by bubble graphs. An adjusted p-value < 0.05 was considered significant. (E) PPI network. Node size indicates the number of proteins that interact with it. The green node represents downregulated proteins, and the red node represents upregulated proteins. Edge width indicates the strength of the interaction between the two proteins. (F) The node protein clusters with the highest scores were constructed by the MCODE plug-in in Cytoscape. (G) The top 15 hub proteins were constructed by cytoHubba. The figure shows the top 15 hub genes constructed by the MCC method. (H) The common hub proteins calculated by seven algorithms of plug-in cytoHubba and their coexpressed genes were analyzed via GeneMANIA. (I) Relative expression of common hub proteins in DEPs between healthy young individuals and healthy elderly individuals