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Application of sector protein microarrays to clinical samples

Clinical Proteomics volume 1, pages91–99(2004)Cite this article

Abstract

Many protein functions are conferred by posttranslational modifications, which allow proteins to perform specific cellular tasks. Protein microarrays enable specific detection of posttranslational modifications not attainable by gene arrays. Reverse-phase protein microarrays have been widely adopted for use with clinical biopsy specimens because they have many advantages including highly reproducible printing of cellular lysates onto array surfaces, buit-in dilution curves, and direct detection using one antibody per analyte. This results in high-sensitivity, broad dynamic range, and favorable precision. Reverse-phase arrays have been restricted to a one slide/one antibody format. Although this is suitable for analyzing treatment effects over populations of samples, it is not well suited to individual patient assessments. One means of reaching this goal is the sector array format. Through the sector array, multiple antibody probes can be multiplexed on a single slide containing replicate immobilized aliquots from one patient. Thus, on one slide, a complete set of analytes can be characterized and used to support a therapy decision. This article describes a method for constructing sector arrays and demonstrates feasibility and adequate sensitivity applied to apoptosis related pathways.

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Affiliations

  1. Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, FDA-NCI Clinical Proteomics Program, Bethesda, MD
    • Virginia Espina
    • , Lance A. Liotta
    •  & David Geho
  2. Center for Biologics Evaluation and Research, Office of Tissue, Cell and Gene Therapy, Food and Drug Administration, FDA-NCI Clinical Proteomics Program, Bethesda, MD
    • Emanuel F. Petricoin III
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  1. Virginia Espina
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  2. Emanuel F. Petricoin III
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  3. Lance A. Liotta
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Correspondence to Virginia Espina.

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Espina, V., Petricoin, E.F., Liotta, L.A. et al. Application of sector protein microarrays to clinical samples. Clin Proteom 1, 91–99 (2004). https://doi.org/10.1385/CP:1:1:091

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Key Words

  • Protein microarray
  • molecular profiling
  • individual targeted
  • sector arrays
  • clinical analysis

Clinical Proteomics

ISSN: 1559-0275